Bacterial-Annotator GEM
Ruby GEM to annotate bacterial genomes based on a reference genome and also complete the annotation with external or remote databases.
To install the gem locally with this package
gem build bacterial-annotator.gemspec
gem install bacterial-annotator-0.0.1.gem
bacterial-annotator -h
Or you could simply install it from rubygems.org with that command
gem install bacterial-annotator
The blat aligner and the prodigal ORF finder will be installed as dependencies if you accept their licenses.
Example of usage
- Bacterial genome annotation
bacterial-annotator annotate -i my_genome.fasta -g reference_genome.gbk -o annotation_output_dir --pidentity 80 --pcoverage 90
# reference_genome.gbk could also only be the genome ID which will be fetched from NCBI
- Bacterial genome comparison
# in a folder where you want to compare diffente genome annotations
bacterial-annotator compare --refgenome reference_genome.gbk --pidentity 80 --align prot --phylogeny --software fasttree *.gbk