Project

bio-cigar

0.0
No commit activity in last 3 years
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A parser for CIGAR format alignments.
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 Popularity
Downloads
7,408
Stars
5
Forks
0
Watchers
3
 Releases
Current version
0.1.0
Total releases
2
First release
2014-02-27
Latest release
2014-02-28
 Issues
Open Issues
0
Closed Issues
2
Total Issues
2
Issue Closure Rate
100%
 Development
Primary Language
Ruby
Licenses
MIT
Average date of last 50 commits
2013-12-16
Reverse Dependencies
0

 Dependencies

Development

bio
>= 1.4.2
bundler
>= 1.0.21
jeweler
>= 0
rdoc
>= 3.12
rspec
>= 2.8.0

Runtime

bio-samtools
>= 0.6.2
 Project Readme

bio-cigar

Build Status

Parser for the cigar sequence alignment format.

Note: this software is under active development!

Installation

gem install bio-cigar

Usage

require 'bio-cigar'

# An example from http://davetang.org/wiki/tiki-index.php?page=SAM
# CIGAR: 3S8M1D6M4S
cigar_string = '3S8M1D6M4S'
cigar = Bio::Cigar.new(cigar_string) #=> Bio::Cigar object

# REF:     TCGCCCGTCTAGCATACGC
# READ: gggGTGTAACC-GACTAGgggg
# MATCH:---001000000010010----
ref =   'TCGCCCGTCTAGCATACGCCCGTCTAGCATACGC'
query = 'gggGTGTAACCGACTAGgggg'
answer = cigar.percent_identity(ref, query)
answer.should == [
  20.0, #20% Identity
  3, #3 matches
  12 #12 mismatches
]

It can also be used directly on SAM format alignments (Bio::DB::Alignment objects), which are produced by bio-samtools:

sam = Bio::DB::Alignment.new
sam.cigar = '196M54S'
sam.pos = 1150
sam.seq = 'ACTGCCGGTGTTAAACCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATGCCCAGGGCTACACACGTGCTACAATGGCCGTTACAAAGCGTCGCTAACCCGCGAGGGGGAGCCAATCGCAAAAAAGCGGCCTCAGTTCAGATTGCAGTCTGCAACTCGACTGCATGAAGTTGGAATCCCTAGTAATCGCGTGTCATTAGCGCGCGGTGAATACGTCCCTGCTCCTTGCACTCACCGCCCGT'
ref = 'GAGCGAACGTTAGCGGCGGGCTTAACACATGCAAGTCGAACGAGAATGAAGGAGCAATCCTTCTAGTAAAGTGGCGGACGGGTGCGTAACACGTGGATAATCTACCTTCCGGCGGGGGACAACAGTTCGAAAGGACTGCTAATACCGCGTACGTCGGCGAGAGCTCAGGCTCTTGTCGGGAAAGATGGCCAATCCTTGGAAGCTGTCACCGGAAGATGAATCCGCGGCCCATCAGGTAGTTGGTGAGGTAATGGCTCACCAAGCCTAAGACGGGTAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGCGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCCACGCCGCGTGAGTGATGAAGGCCTTCGGGTCGTAAAGCTCTGTGGGGAGGGACGAACAAGTGCGTATCGAATAAATACGTGCCCTGACGGTACCTCCTTAGCAAGCACCGGCTAACCATGTGCCAGCAGCCGCGGTAATACATGGGGTGCAAACGTTGCTCGGAATTATTGGGCGTAAAGCGCGCGTAGGCGGTCGCTTAAGTCGGATGTGAAATCCCTCGGCTTAACTGAGGAAGTGCATCCGAGACTGAATGGCTAGAGTACGAAAGAGGGTCGNNNNNTTCCCGGTGTAGAGGTGAAATTCGTAGATATCGGGAGGAACACCGGCGGCGAAGGCGGCGACCTGGTTCGAGACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATGCTAGATGTTTCTGGTATTGACCCCGGAGGCGTCGTAGCTAACGCGATAAGCATCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTCAATTTGACGCAACGCGAAGAACCTTACCTGGGTTGGAACCCTCCAGAAGTCCGCAGAGATGTGGATGTGCTCGCAAGAGAACTGGATGTCCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCGTTAGTTGCTAACAGTTCGGCTGAGCACTCTAACGAGACTGCCGGTGTTAAACCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATGCCCAGGGCTACACACGTGCTACAATGGTCGTTACAAAGCGTCGCTAACCCGCGAGGGGGAGCTAATCGCAAAAAAGCGGCCTCAGTTCAGATTGCAGTCTGCAACTCGACTGCATGAAGTTGGAATCGCTAGTAATCCCTGATCAGCAGGCAGGGGTGAATACGTTCCCGGGCC'
sam.percent_identity(ref).should == [
  98.46938775510205,
  193,
  3
]

The API doc is online. For more code examples see the test files in the source tree.

Project home page

Information on the source tree, documentation, examples, issues and how to contribute, see

http://github.com/wwood/bioruby-cigar

The BioRuby community is on IRC server: irc.freenode.org, channel: #bioruby.

Cite

This software is currently unpublished.

Biogems.info

This Biogem is published at (http://biogems.info/index.html#bio-cigar)

Copyright

Copyright (c) 2013 Ben J Woodcroft. See LICENSE.txt for further details.